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Technique for Enumeration of Renibacterium salmoninarum in Fish Kidney Tissues
Author(s) -
Lee Ernie G.H.
Publication year - 1989
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1989)001<0025:tfeosi>2.3.co;2
Subject(s) - biology , kidney , fish <actinopterygii> , microbiology and biotechnology , enumeration , membrane filter , veterinary medicine , fishery , membrane , biochemistry , endocrinology , medicine , mathematics , combinatorics
Abstract Effects of prefiltration and enzyme treatment on the filtrability of kidney tissue of chinook salmon Oncorhynchus tshawytscha were examined. The clarification of kidney suspensions with a 5‐μm prefilter did not affect microbial recovery from the samples examined. Samples of kidney suspensions inoculated with Renibacterium salmoninarum (10 2 ‐10 5 cells/g) were prefiltered and then tested for their filtrability through a 0.2‐μm membrane filter. Less than 50% of the samples could be filtered without enzyme treatment, but all samples were rendered filtrable by enzyme treatment. Inoculum levels of R. salmoninarum as low as 100 cells/g could be detected and quantified. The application of these procedures greatly enhances membrane filtration for the certification of R. salmoninarum ‐free fish.