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Comparison of Methods Used to Detect Renibacterium salmoninarum, the Causative Agent of Bacterial Kidney Disease
Author(s) -
Sakai M.,
Atsuta S.,
Kobayashi M.
Publication year - 1989
Publication title -
journal of aquatic animal health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 52
eISSN - 1548-8667
pISSN - 0899-7659
DOI - 10.1577/1548-8667(1989)001<0021:comutd>2.3.co;2
Subject(s) - biology , microbiology and biotechnology , immunoassay , latex fixation test , antigen , antibody , immunology
Various diagnostic methods used to detect Renibacterum salmoninarum , the causative agent of bacterial kidney disease (BKD), were compared. The most sensitive method was the enzyme immunoassay (the indirect dot blot assay), which could detect 10 2 bacterial cells per gram of kidney tissue. The minimum bacteria concentrations showing positive reactions to the indirect fluorescent antibody test (IFAT) and the coagglutination test were 10 3 and 10 4 cells/g kidney, respectively. The sensitivities of the Gram stain, immunodiffusion procedure, and latex agglutination test were low, and these methods could only be applied to fish with overt BKD symptoms. Altogether, 656 coho salmon Oncorhynchus kisutch were examined for R. salmoninarum antigen with the direct and indirect dot blot assays (DDBA and IDBA) and the IFAT. Among the fish sampled, positive reactions were obtained in 11.8% by the DDBA, 28.2% by the IDBA, and 12.9% by the IFAT.