Premium
Microsatellite Analysis of Genetic Variation in Sturgeon: New Primer Sequences for Scaphirhynchus and Acipenser
Author(s) -
McQuown Eve C.,
Sloss Brian L.,
Sheehan Robert J.,
Rodzen Jeff,
Tranah Gregory J.,
May Bernie
Publication year - 2000
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(2000)129<1380:maogvi>2.0.co;2
Subject(s) - sturgeon , microsatellite , acipenser , biology , lake sturgeon , endangered species , zoology , genetics , primer (cosmetics) , genetic variation , genetic marker , evolutionary biology , allele , fishery , ecology , gene , habitat , chemistry , organic chemistry , fish <actinopterygii>
Low levels of genetic variation at traditional molecular markers have hampered genetic research within the family Acipenseridae. In an effort to develop a large set of polymorphic genetic markers, 172 clones were sequenced from three subgenomic libraries of shovelnose sturgeon Scaphirhynchus platorynchus ; the libraries were enriched for two dinucleotide and one tetranucleotide microsatellite motifs (CA, GA, and TAGA). Primers were designed for 113 of the sequences and tested against shovelnose sturgeon, pallid sturgeon S. albus , white sturgeon Acipenser transmontanus , lake sturgeon A. fulvescens , and green sturgeon A. medirostris. Of the 113 primer sets tested, 96% amplified in one or more species (58 dimeric and 50 tetrameric). In Scaphirhynchus species, 93% of all loci amplified, and 76% were polymorphic. Within the individual Acipense r species, 65–80% of loci amplified, with 42–58% being polymorphic. Polymorphic systems for Scaphirhynchus species predominately displayed simple, disomic banding patterns, while those for Acipenser species typically displayed banding patterns characteristic of tetraploid or higher polyploid levels. These new microsatellite loci provide a group of genetic markers that are detectable with noninvasive sampling and that should prove useful in the preservation of threatened and endangered sturgeon species worldwide.