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Comparison of Control Region Sequencing and Fragment RFLP Analysis for Resolving Mitochondrial DNA Variation and Phylogenetic Relationships among Great Lakes Walleyes
Author(s) -
Gatt Michael H.,
Ferguson Moira M.,
Liskauskas Arunas P.
Publication year - 2000
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(2000)129<1288:cocrsa>2.0.co;2
Subject(s) - restriction fragment length polymorphism , phylogenetic tree , haplotype , biology , mitochondrial dna , genetics , mega , restriction site , mtdna control region , fragment (logic) , restriction fragment , evolutionary biology , genetic variation , polymerase chain reaction , restriction enzyme , dna , gene , genotype , physics , astronomy , computer science , programming language
Direct sequencing of 513 base pairs from the control region and restriction fragment length polymorphisms (RFLP) in two fragments totaling 7.6 kilobases (fragment RFLP) that were amplified by polymerase chain reaction were used to assess mitochondrial DNA (mtDNA) variation in Great Lakes walleye Stizostedion vitreum. Our objective was to determine the effectiveness of these mtDNA markers in detecting genetic variation and resolving phylogenetic relationships among haplotypes previously identified by RFLP analysis of the entire molecule. The fragment RFLP analysis surveyed 554 base pairs and detected almost twice as many haplotypes as did the sequencing analysis. However, both approaches resulted in similar tree topologies and resolved the three major phylogenetic assemblages published in entire‐molecule RFLP studies.