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A Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR–RFLP) Assay for the Discrimination of Mitochondrial DNA from the Florida and Northern Subspecies of Largemouth Bass
Author(s) -
Bremer Jaime R. Alvarado,
Zhang Liang,
Bulak James S.,
Ely Bert
Publication year - 1998
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1998)127<0507:apcrnr>2.0.co;2
Subject(s) - subspecies , restriction fragment length polymorphism , mitochondrial dna , biology , haplotype , bass (fish) , polymerase chain reaction , restriction site , restriction enzyme , micropterus , genetics , dna , zoology , ecology , gene , genotype
Distinct mitochondrial DNA haplotypes, based on the presence or absence of a Taq I restriction site in the control region, distinguished largemouth bass Micropterus salmoides of the northern subspecies ( N = 62) from the Florida subspecies ( N = 28). The presence of either northern ( N = 6) or Florida ( N = 3) haplotypes in Lake Wateree, South Carolina, suggests that these haplotypes will be particularly useful in detecting the coexistence of these subspecies.