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Improved Method for the Initiation of Continuous Cultures of the Oyster Pathogen Perkinsus marinus (Apicomplexa)
Author(s) -
La Peyre Jerome F.,
Faisal Mohamed
Publication year - 1995
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1995)124<0144:imftio>2.3.co;2
Subject(s) - crassostrea , oyster , biology , apicomplexa , pathogen , eastern oyster , protozoa , mass culture , zoology , microbiology and biotechnology , fishery , protozoal disease , immunology , sociology , malaria , anthropology
A simple method for initiating continuous cultures of Perkinsus marinus with hypnospores is described. The visceral mass of an infected eastern oyster Crassostrea virginica was first incubated in Rayˈs fluid thioglycollate medium in order to produce large hypnospores from the smaller histozoic stages of the protozoan. Hypnospores were then purified and transferred into the culture medium JL‐ODRP‐1 where, within a relatively short period of time, they further divided by either progressive cleavage or successive bipartition. These divisions produced large numbers of merozoites and flagellated cells. This two‐step procedure surpassed the previously described method of initiating cultures from hearts of infected eastern oysters because a much higher number of purified, larger P. marinas cells could be obtained from a single infected eastern oyster.