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Differentiation of Mitochondrial DNA in Largemouth Bass
Author(s) -
Nedbal Michael A.,
Philipp David P.
Publication year - 1994
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1994)123<0460:domdil>2.3.co;2
Subject(s) - subspecies , biology , bass (fish) , nucleotide diversity , mitochondrial dna , micropterus , restriction fragment length polymorphism , haplotype , genetics , zoology , ecology , polymerase chain reaction , gene , genotype
Abstract Mitochondrial DNA (mtDNA) variation was examined for two nominal subspecies of largemouth bass, Micropterus salmoides salmoides (northern largemouth bass) and M. s. floridanus (Florida largemouth bass). Mitochondrial DNA nucleon diversity values within samples ranged from 0.378 to 0.911; the value over all individuals surveyed was 0.917. Estimated percentage nucleotide sequence divergence among the 19 haplotypes ranged from 0.081 to 5.80 (mean ± SE, 2.72 ± 0.459). The average percentage nucleotide sequence divergence within populations ranged from 0.137 to 0.526 (mean ± SE, 0.312 ± 0.067). The average percentage nucleotide sequence divergence among pairwise comparisons of populations ranged from 0.174 to 5.11 (mean ± SE, 2.91 ± 0.523). Parsimony and phenetic analysis of populations yielded strong differentiation between the two subspecies and provided enough resolution to discriminate among populations within the same subspecies. These data suggest that restriction fragment length polymorphism (RFLP) analysis of mtDNA may provide greater resolution than protein electrophoresis for stock identification among largemouth bass populations. The large number of unique haplotypes found may also be useful in evaluating the success of stocking programs.