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Natural Occurrence of Triploidy in a Wild Brown Bullhead
Author(s) -
Cormier Susan M.,
Neiheisel Timothy W.,
Williams Daniel E.,
Tiersch Terrence R.
Publication year - 1993
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1993)122<0390:nootia>2.3.co;2
Subject(s) - ictalurus , biology , ploidy , flow cytometry , dna , cytometry , zoology , microbiology and biotechnology , genetics , fish <actinopterygii> , fishery , gene , catfish
We report the rare, natural occurrence of triploidy in a brown bullhead Ameiurus (formerly Ictalurus ) nebulosus . This is to our knowledge the first report of naturally occurring triploidy within the family Ictaluridae. The nuclei of blood cells were studied by flow cytometry, volume measurement, and computer‐assisted image analysis. The triploid had a genome size of 3.29 pg DNA/cell compared to 2.23 pg DNA/cell (SD, 0.02 pg) for 28 diploid brown bullheads. Volume measurement and image analysis were able to detect the triploid condition; however, flow cytometry was the most precise technique and had the greatest resolution.