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Two Cell Lines from White Sturgeon
Author(s) -
Hedrick Ronald P.,
McDowell Terry S.,
Rosemark Renee,
Aronstein Diane,
Lannan Catharine N.
Publication year - 1991
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1991)120<0528:tclfws>2.3.co;2
Subject(s) - biology , iridovirus , sturgeon , virus , virology , infectious hematopoietic necrosis virus , cell culture , infectious pancreatic necrosis virus , viral replication , embryonated , cytopathic effect , rainbow trout , genetics , fish <actinopterygii> , fishery
Cell lines were established from spleen and heart tissues of a juvenile white sturgeon Acipenser transmontanus and designated WSS‐2 and WSH‐1, respectively. Isoenzyme analyses of both lines confirmed their host of origin. Both lines were hypotetraploid and possessed micro‐ and macrochromosomes. The modal chromosome number for the WSS‐2 line was 219; the WSH‐1 line had a bimodal distribution with modes of 237 and 243. The effects of temperature on growth and susceptibility to selected viral pathogens were examined for the WSS‐2 line. Cell replication occurred at 15–30°C, optimum temperature was 25°C. Cell survival was observed after 11 d at 10°C but not at 35°C. The WSS‐2line was refractory to infections with channel catfish virus (CCV) and infectious pancreatic necrosis virus (IPNV) but susceptible to infectious hematopoietic necrosis virus (IHNV) and white sturgeon iridovirus (WSIV). The need for cell lines from the host of origin in studies of viral pathogens of fish was demonstrated by the ability of the WSS‐2 line to support replication of WSIV.