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DNA Fingerprints in Rainbow Trout Detected by Hybridization with DNA of Bacteriophage M13
Author(s) -
Fields Robert D.,
Johnson Kenneth R.,
Thorgaard Gary H.
Publication year - 1989
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1989)118<0078:dfirtd>2.3.co;2
Subject(s) - salmo , biology , rainbow trout , restriction enzyme , southern blot , genomic dna , genetics , dna , dna profiling , restriction fragment length polymorphism , bacteriophage , microbiology and biotechnology , zoology , fish <actinopterygii> , fishery , gene , polymerase chain reaction , escherichia coli
Abstract We used radiolabeled DNA of bacteriophage M13 to probe Southern blots of restriction enzyme‐digested genomic DNA of rainbow trout Oncorhynchus mykiss . We found a high level of polymorphism for restriction fragment lengths by analysis of DNA from a single‐pair mating between individuals of an outbred strain of this species. Each of the offspring of this mating exhibited a unique banding pattern. Similar polymorphic patterns were found in chum salmon O. keta , coho salmon O. kisutch , and Atlantic salmon Salmo salar . This technique shows great promise for demographic analysis and unabiguous genetic tagging of individual fish.