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Comparison of Five Techniques for the Detection of Renibacterium salmoninarum in Adult Coho Salmon
Author(s) -
Pascho R. J.,
Elliott D. G.,
Mallett R. W.,
Mulcahy D.
Publication year - 1987
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1987)116<882:coftft>2.0.co;2
Subject(s) - counterimmunoelectrophoresis , biology , immunodiffusion , antibody , spleen , microbiology and biotechnology , immunology
Samples of kidney, spleen, coelomic fluid, and blood from 56 sexually mature coho salmon Oncorhynchus kisutch were examined for infection by Renibacterium salmoninarum by five methods. The overall prevalence (all sample types combined) of R. salmoninarum in the fish was 100% by the enzyme‐linked immunosorbent assay, 86% by the combined results of the direct fluorescent antibody and the direct filtration‐fluorescent antibody techniques, 39% by culture, 11% by counterimmunoelectrophoresis, and 5% by agarose gel immunodiffusion. There was a significant positive correlation (P < 0.001) between the enzyme‐linked immunosorbent assay absorbance levels and the counts by fluorescent antibody techniques for kidney, spleen, and coelomic fluid, and significant positive correlations (P < 0.001) in enzyme‐linked immunosorbent assay absorbance levels for all four of the sample types.