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Evaluation of Radioimmunoassay as a Method to Quantify Trypsin and Trypsinogen in Fish
Author(s) -
Hjelmeland Knut,
Jørgensen Trond
Publication year - 1985
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1985)114<619:eoraam>2.0.co;2
Subject(s) - trypsinogen , radioimmunoassay , trypsin , fish <actinopterygii> , chemistry , enzyme , chromatography , biochemistry , biology , fishery
Abstract A radioimmunoassay of trypsin and trypsinogen in fish has been developed. The antibodies detect both trypsin and trypsinogen, and the presence of trypsin inhibitors in the assay extract does not affect the readings. The radioimmunoassay, used in combination with conventional enzyme assay, is very suitable for mechanistic studies of the production and activation of trypsin in fish.