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Cryogenic Preservation of Sperm from Striped Bass
Author(s) -
Kerby Jerome Howard
Publication year - 1983
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1983)112<86:cposfs>2.0.co;2
Subject(s) - bass (fish) , sperm , fishery , zoology , biology , botany
Spermatozoa of striped bass Morone saxatilis were successfully cryopreserved, retaining fertilizing capacity for up to 2 years. Spermatozoa in 14 extenders, in combination with three concentrations of four cryoprotectants at various sperm : medium ratios, were frozen at different rates and tested. Best results were obtained with extender “OH‐189” (7.30 g NaCl, 0.38 g KCl, 0.23 g CaCl 2 ˙2H 2 O, 5.00 g NaHCO 3 , 0.41 g NaH 2 PO 4 ˙H 2 O, 0.23 g MgSO 4 ˙7H 2 O, 5.00 g fructose, 7.50 g lecithin, and 5.00 g mannitol in 968.95 g distilled H 2 O) combined with 5.0% dimethylsulfoxide and mixed in a 1:4 sperm : medium volume ratio. The highest fertilization percentage obtained with cryopreserved semen was 87.7. No fertilization was obtained when glycerol, ethylene glycol, or propylene glycol were the cryoprotectants. Mean freezing rates faster than 5 C/minute were more effective than slower rates.