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14 C‐Glycine Uptake by Fish Scales: Refinement of a Growth Index and Effects of a Protein‐Synthesis Inhibitor
Author(s) -
Goolish Edward M.,
Adelman Ira R.
Publication year - 1983
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1983)112<647:cubfs>2.0.co;2
Subject(s) - glycine , fish <actinopterygii> , index (typography) , chemistry , biology , biochemistry , amino acid , fishery , computer science , world wide web
In vitro 14 C‐glycine uptake by the scales of bluegill Lepomis macrochirus was sensitive to the pH of the incubation medium; maximum uptake occurred at approximately pH 7.7. Cycloheximide, an inhibitor of protein synthesis, reduced glycine uptake by up to 90%, providing further evidence for the involvement of glycine uptake in the process of protein synthesis. The minimum duration of handling stress that resulted in a significant change in glycine uptake was 60 minutes; after 240 minutes of continuous stress, glycine uptake was reduced 62% from control values. Diel sampling of laboratory fish indicated no daily rhythm in glycine uptake and demonstrated a significant correlation (r = 0.837) between glycine uptake and fish growth rate as estimated over a 2‐week interval. The results of these experiments suggest that glycine uptake by scales can be a reliable index of fish growth or state of stress. Received December 18, 1982 Accepted June 12, 1983