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Biochemical Genetic Analyses of the Grass Carp ♀ X Bighead Carp ♂ F 1 Hybrid and the Parental Species
Author(s) -
Magee Sheila M.,
Philipp David P.,
Philipp David P.
Publication year - 1982
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1982)111<593:bgaotg>2.0.co;2
Subject(s) - grass carp , bighead carp , biology , hybrid , hypophthalmichthys , carp , allele , introgression , isozyme , heterosis , zoology , genetics , botany , gene , silver carp , fishery , fish <actinopterygii> , enzyme , biochemistry
The patterns of isozyme expression in tissues of the grass carp Ctenopharyngodon idella, bighead carp Hypophthalmichthys nobilis, and F 1 hybrid (grass carp ♀ x bighead carp ♂) were analyzed by vertical starch gel electrophoresis and histochemical staining procedures. Both parental species and the F 1 hybrid were fixed for electrophoretically indistinguishable alleles at 21 of 30 loci studied. However, the two parental species were fixed for different alleles at the remaining nine loci. Quantitative isozyme determinations were used to calculate the parental gene dosages in individuals of the 1979, 1980, and 1981 broods of F 1 hybrids produced in Arkansas. Exclusive of low percentages of gynogenetic grass carp observed, the percentages of triploid F 1 hybrids were 100% (1979), 44% (1980), and 100% (1981).