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Bioconcentration of 2,2′,4,4′‐Tetrachlorobiphenyl in Rainbow Trout as Measured by an Accelerated Test
Author(s) -
Branson D. R.,
Blau G. E.,
Alexander H. C.,
Neely W. B.
Publication year - 1975
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1975)104<785:botirt>2.0.co;2
Subject(s) - bioconcentration , rainbow trout , salmo , trout , fish <actinopterygii> , chemistry , steady state (chemistry) , zoology , fishery , biology , environmental chemistry , bioaccumulation
Some hydrophobic chemicals may reach plateau levels in fish only after several months of continuous exposure. Therefore, an accelerated test procedure, based on kinetics, was developed using an isomer of PCBˈs (polychlorinated biphenyls): 2,2′,4,4′‐tetrachlorobiphenyl. The rates of uptake and clearance of 2,2′,4,4′‐tetrachlorobiphenyl were determined by analysis of rainbow trout (Salmo gairdneri Richardson). These trout were exposed to 1.6 and 9.0 μg/liter for five days and then transferred to fresh water. A nonlinear regression analysis was used to estimate the rate constants for uptake and clearance, and the bioconcentration factor at steady‐state was calculated from the rate constants. For 2,2′,4,4′‐tetrachlorobiphenyl, the bioconcentration factor at steady‐state was 9550 ± 1610 in trout muscle. The accuracy of the bioconcentration factor determined by this accelerated test procedure was compared with experimental observations in a 42‐day test. The concentration of 2,2′,4,4′‐tetrachlorobiphenyl in trout muscle was 82 ± 20 μg/g after 42 days continuous exposure to 14 μg/liter. This was in good agreement with 92 ± 18 μg/g predicted from the accelerated procedure. The 42‐day level was about 43% of the theoretical steady‐state for 2,2′,4,4′‐tetrachlorobiphenyl in trout muscle. Therefore, this accelerated procedure can provide information about the potential of a chemical to bioconcentrate in fish in a much shorter period of time than can be provided by previously described methods.