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Some Physicochemical Properties of Transferrins in Brook Trout
Author(s) -
Hershberger William K.
Publication year - 1970
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1970)99<207:sppoti>2.0.co;2
Subject(s) - transferrin , trout , chemistry , zoology , fishery , biology , fish <actinopterygii> , biochemistry
Three brook trout transferrins, designated A, B and C, were purified by use of rivanol precipitation and gradient elution chromatography with DEAE‐Cellulose. Physical and chemical analyses of the purified transferrins demonstrated that each type had at least four sialic acids attached to the protein moiety and that the A type of transferrin has a number of amino acid substitutions, as shown by amino acid analysis and fingerprinting. No differences could be shown between the B and C types. Analyses of molecular weights were inconclusive. Studies on the total iron‐binding capacity of the transferrins suggested a complementarity between the proteins when in heterozygous condition. The functional and genetic bases for the lethality of the AA homozygotes are discussed.