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Spontaneous Neoplastic Transformation of the Gill Cell Line FG‐9307 from the Olive Flounder Paralichthys olivaceus
Author(s) -
Guo Huarong,
Zhang Shicui,
Li Hongyan
Publication year - 2003
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1577/1548-8454(2003)065<0044:sntotg>2.0.co;2
Subject(s) - paralichthys , biology , olive flounder , flounder , neoplastic transformation , gill , transformation (genetics) , cell culture , in vitro , fish <actinopterygii> , microbiology and biotechnology , fishery , cancer , biochemistry , carcinogenesis , gene , genetics
The present study reports that FG‐9307 cells derived from the gills of the olive flounder Paralichthys olivaceus underwent neoplastic transformation spontaneously during the process of in vitro culture. Cells lost their density‐dependent inhibition and contact inhibition properties, and they acquired genomic variation and the capacity of piling up in a focus and growing in semisolid agar. This is the first report of in vitro spontaneous neoplastic transformation of fish cell lines. The preneoplastically and neoplastically transformed FG cells provide a powerful tool to screen carcinogenic and anticancer agents in the aquatic environment.