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Investigations in Concurrent Disinfection and De‐adhesion of Lake Sturgeon Eggs
Author(s) -
Bouchard Henry J.,
Aloisi Douglas B.
Publication year - 2002
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1577/1548-8454(2002)064<0212:iicdad>2.0.co;2
Subject(s) - tannic acid , distilled water , adhesion , iodine , urea , biology , lake sturgeon , zoology , sturgeon , acipenser , chemistry , fish <actinopterygii> , fishery , botany , biochemistry , chromatography , organic chemistry
Recent interest in the restoration of lake sturgeon Acipenser fulvescens and in the development of new culture techniques has led to many new management programs designed to stabilize and enhance existing wild populations. The spread of pathogens affecting both wild and propagated sturgeon is a major concern for restoration programs. In 1999, two methods of egg de‐adhesion were used on two separate groups of lake sturgeon eggs prior to disinfection with 100 mg/L of iodine for 10 min. The de‐adhesion of eggs with urea−NaCl–tannic acid solutions produced hatch and 14‐d‐posthatch survival rates of 77.2% and 71.0%, respectively. These results were compared with those for eggs treated with Fuller's Earth (control), which had survival rates of 88.0% and 86.0%, respectively, at hatch and 14 d. In 2000, we compared three methods of de‐adhesion: (1) a solution of distilled water (jelly inhibitor) and iodine, (2) a solution of urea, NaCl, and tannic acid, and (3) a solution of Fuller's Earth and iodine. With all three methods, eggs were disinfected with a 50‐mg/L buffered iodine solution for 30 min during or immediately following de‐adhesion. Survival to hatchout for the distilled water, urea−NaCl−tannic acid, and Fuller's Earth methods was 95.3, 99.6, and 97.7%, respectively. Survival to 14 d posthatch was 76.8, 84.2, and 85.6%, respectively, for the three methods. Iodine disinfection during the de‐adhesion and water absorption processes does not appear to be toxic to lake sturgeon eggs. Topical iodine disinfection of lake sturgeon eggs immediately (<10 min) following fertilization increased the exposure of both the internal and external portions of the egg to the iodine during the hydration of the egg envelope.