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Ploidy Level Determination in Genetically Manipulated Northern Pike Based on the Number of Active Nucleoli per Cell
Author(s) -
Kucharczyk Dariusz,
Woznicki Pawel,
Luczynski Marek J.,
Klinger Marek,
Luczynski Miroslaw
Publication year - 1999
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1577/1548-8454(1999)061<0038:pldigm>2.0.co;2
Subject(s) - ploidy , biology , pike , nucleolus , esox , fish <actinopterygii> , andrology , nucleus , microbiology and biotechnology , genetics , fishery , gene , medicine
Silver‐staining of cell preparations followed by counting of the mean number of active nucleoli per cell enabled determination of the ploidy level in genetically manipulated northern pike Esox lucius. Haploid pike resulted from activation of eggs with sperm irradiated with ultraviolet light, and triploid specimens were obtained by applying high‐temperature shock. Cells of haploid individuals had one (almost 100% of cells examined) or, sporadically, two nucleoli. Depending on the specimen, mean number of nucleoli per nucleus ranged from 1.0 to 1.06. In diploid fish, the mean number of active nucleoli per cell ranged from 1.58 to 1.81. In triploids, the mean ranged from 2.08 to 2.46. Positive identification of ploidy level in northern pike required examination of up to 40 cells per individual, as determined by the regression analysis. The method required a very small amount of any kind of tissue, enabling determination of ploidy level in juvenile fish without sacrificing them.