Open AccessDEVELOPMENT OF THE QUANTITATIVE IMMUNOCHROMATOGRAPHY TESTS FOR SOMATIC DEASEASE MARKERS DETECTION
Author(s) -
С. И. Третьяков,
L. A. Basharova,
С П Ярков,
A A Titiov,
Е Н Храмов
Publication year - 2013
Publication title -
vestnik rossijskoj akademii medicinskih nauk
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.122
H-Index - 15
eISSN - 2414-3545
pISSN - 0869-6047
DOI - 10.15690/vramn.v68i9.780
Subject(s) - immunoassay , analyte , chromatography , fibrin , chemistry , d dimer , immunoglobulin e , antibody , medicine , immunology , gastroenterology
The purpose of work was creation of quantative immunochromatographic tests (ICT) for measuring concentrations of the marker substance associated with somatic diseases: immunoglobulin Е (IgE), C-reactive protein (CRP) and fibrin D-dimer in blood serum (plasma), which is carried out with the help of videodigital analyzers of domestic development "Reflecom" and "Zondaj". ICT were designed in "sandwich» format, using colloid gold and monoclonal antibodies. It is shown, that calibration curves received with the help of ICT and devices of videodigital registration, are well approximated by exponential dependence Y=Aexp(-x/B)+y0, where Y- device readings , х- analytes concentration, А, В, у0– constants. Samples of blood serum (plasma), under investigation received in conditions of a hospital from patients. Correlation with electrochemiluminescent immunoassay, enzyme linked immunoassay, latex-agglutinations assay and immunochromatographic method was observed. Sensitivity of the developed tests was- 7,5 IU/ML for IgE, 5,7 ng/ml for CRP, 500 ng/ml for fibrin D-dimer. The developed analytical complex- videodigital analyzer "Zondaj" and ICT for quantitative measure of concentration IgE, fibrin D-dimer and CRP- can be successfully applied in laboratory practice and clinical laboratory researches alongside with actual immunochemistry methods.