Open AccessAPOPTOSIS CELLS OF CORONARY ARTERY WALL AS DEVELOPING AND PROGRESSING FACTOR OF CORONARY SCLEROSIS
Author(s) -
Vladimirskaia Te,
Shved Ia,
Krivorot Sg
Publication year - 2013
Publication title -
vestnik rossijskoj akademii medicinskih nauk
Language(s) - English
Resource type - Journals
eISSN - 2414-3545
pISSN - 0869-6047
DOI - 10.15690/vramn.v68i9.774
Subject(s) - tunel assay , coronary arteries , artery , medicine , apoptosis , pathology , fibrous cap , necrosis , h&e stain , vascular smooth muscle , coronary atherosclerosis , coronary artery disease , cardiology , smooth muscle , staining , immunohistochemistry , biology , biochemistry
Objective: to study apoptosis of individual cellular components of the vascular wall of coronary arteries at different morphological stages of atherosclerosis. Material and methods. The study was performed on coronary arteries taken from 52 deceased patients with atherosclerosis and coronary heart disease at different stages of atherogenesis. For morphological study prepared paraffin sections, which were stained for morphological studies were prepared paraffin sections, which were stained with hematoxylin and eosin, by Van Gieson, Masson, on lipids with Sudan black B, according to Van Cossu. .To determine apoptosis, TUNEL method used in paraffin sections. Apoptotic index (AI) was calculated by TUNEL-positive cells and the average inner shell coronary artery around the perimeter each with increasing microscopic 1000. Results. Investigation showed significant apoptosis (p 0.05) increase in AI smooth muscle, endothelial cells, macrophages in the coronary arteries affected by atherosclerosis compared to intact control group vascular segments significant reduction AI endothelial, smooth muscle cells and macrophages (p 0,05) traced from the early stages of atherogenic disorders to atheromatosis. Conclusions. It is established that apoptosis of smooth muscle cells, macrophages and endothelial cells is the most intensive on early stages of atherosclerotic process. In process of progressing of atherosclerosis intensity and prevalence of apoptosis of coronary artery wall cells decreases, and processes of necrosis becomes predominant. Apoptosis of coronary artery wall cells is valuable in increasing the zones of atheromatosis, plaque destabilizations, and also increases the risk of thrombosis and ulcerations.