Open AccessINFLUENCE OF REGULATORY T CELLS ON THE FUNCTIONING OF NATURAL KILLER CELLS DURING CANCER IMMUNOTHERAPY
Author(s) -
Irina O. Chikileva,
И. Ж. Шубина,
E. V. Kiselevskii
Publication year - 2012
Publication title -
annals of the russian academy of medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2414-3545
pISSN - 0869-6047
DOI - 10.15690/vramn.v67i4.201
Subject(s) - il 2 receptor , foxp3 , cytotoxic t cell , peripheral blood mononuclear cell , interleukin 21 , immunotherapy , cancer immunotherapy , immunology , natural killer t cell , interleukin 12 , lymphokine activated killer cell , interleukin 2 , chemistry , biology , microbiology and biotechnology , cancer research , cytokine , t cell , immune system , in vitro , biochemistry
One of the common arguments against cancer immunotherapy based on natural killer (NK) cells activated in the presence of interleukin-2 (IL-2) is the probability of the activation of regulatory T cells (Tregs) by IL-2 besides NK cells. Thus, we have monitored numbers of FoxP3+CD4+CD25+ T cells in the samples of healthy volunteers’ peripheral blood mononuclear cells (PBMCs) cultured with or without IL-2. We observed marked increase in the percentages of the CD4+CD25+ T cells in the presence of IL-2. Proportions of Foxp3+CD4+CD25+ T cells feebly increased, remained on the same level or even decreased compared to PBMCs cultured without exogenous IL-2. Based on the absence of FoxP3 expression, most of the CD4+CD25+ T cells purified from IL-2 activated PBMCs were not Tregs, but activated Th cells. Moreover, the addition of the purified supposed Tregs to samples of activated NK cells never inhibited their cytotoxic reactions.
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