Open AccessInhibition of Staphylococcus aureus enterotoxin genes by using plant extracts
Author(s) -
Srwa Ali Mohammed,
Mohammed I. J. Al-ani,
Dereh Lattif Mohammed,
Lina R. Salar,
Banaz M. Rasul
Publication year - 2018
Publication title -
innovaciencia
Language(s) - English
Resource type - Journals
ISSN - 2346-075X
DOI - 10.15649/2346075x.469
Subject(s) - enterotoxin , staphylococcus aureus , microbiology and biotechnology , biology , cinnamomum zeylanicum , traditional medicine , gene , medicine , bacteria , food science , escherichia coli , biochemistry , genetics , essential oil
Enterotoxigenic Staphylococcus aureus is an important pathogen that causes septicemia and bacteremia and is often associated with serious complications, such as endocarditis and osteomyelitis. Some Staphylococcus enterotoxins require only minute quantities to be toxic in humans. The present study focused on investigation how to remove this problematic issue. Objectives: This study was conducted to inhibit S. aureus enterotoxin genes that obtained from positive blood culture bottles of patients at the pediatric hospital in Sulaimania city. Methods: Twenty five isolates of S. aureus were isolated among 100 positive blood culture bottles and determined the strains that produce enterotoxins through culture method. Then, the enterotoxin genes that located on plasmids were cured by two medicinal plants (Eugenia caryophyllata and Cinnamomum zeylanicum). Results: The results showed that nine out of 25 isolates were released enterotoxins from which the plasmid encoding enterotoxin genes were confirmed in four of them. And, two of the isolates were transferred to recipient DH10B E. coli isolate successfully. Methanol extracts of (E. caryophyllata and C. zeylanicum) were used at sub minimum inhibition concentration as curing agents. Conclusion: Methanol extracts of (E. caryophyllata and C. zeylanicum) have grate effect on eliminating the plasmidsencoding enterotoxin gene of S. aureus.