Open AccessIsolation and characterization of a gene encoding farnesyl diphosphate synthase from \(\textit{Panax vietnamensis}\) Ha et Grushv
Author(s) -
Nguyễn Văn Giảng,
Luu Han Ly,
Phung Thi Thu Hang,
Lê Thị Thu Hiền
Publication year - 2021
Publication title -
academia journal of biology
Language(s) - English
Resource type - Journals
eISSN - 2815-5920
pISSN - 2615-9023
DOI - 10.15625/2615-9023/16356
Subject(s) - biosynthesis , gene , biology , farnesyl diphosphate synthase , biochemistry , triterpene , complementary dna , atp synthase , nucleotide sugar , enzyme , open reading frame , traditional medicine , peptide sequence , medicine , alternative medicine , pathology
Panax vietnamensis Ha et Grushv. is a species of the genus Panax native to Central Vietnam, containing a family of triterpene saponins named ginsenosides. This group of biomolecules possesses valuable therapeutic properties against cancer, hepatitis, diabetes, inflammation as well as stress and anxiety. Farnesyl diphosphate synthase (FPS) is a key enzyme participating in the ginsenoside biosynthesis pathway. In this study, a FPS gene from P. vietnamensis (PvFPS) was isolated and characterized. The PvFPS cDNA contained an open reading frame of 1032 bp, encoding a polypeptide chain of 342 amino acid residues. Nucleotide sequence comparison showed that FPS was highly conserved among most species, with two Aspartate-rich motifs responsible for product chain length determination strongly sustained. PvFPS was closely related to those of the same genera and order and differed from those from other kingdoms. PvFPS expression was detected at a greater level in root tissues than in leaves in all ages. Our findings provided information concerning the properties of a crucial gene in the ginsenoside biosynthesis, thus enhancing our understanding of this important pathway.