Open AccessThe DNA barcodes for the species delimitation of the genus \(\textit{Tylopus}\) jeekel, 1968 in Vietnam (Diplopoda: Polydesmida: Paradoxosomatidae)
Author(s) -
Nguyễn Đức Ánh,
Nguyen Thi Thu Anh,
Phung Thi Hong Luong,
Dang Thi Hoa,
Nguyen Giang Son
Publication year - 2021
Publication title -
academia journal of biology
Language(s) - English
Resource type - Journals
eISSN - 2815-5920
pISSN - 2615-9023
DOI - 10.15625/2615-9023/15761
Subject(s) - millipede , dna barcoding , intraspecific competition , biology , phylogenetic tree , myriapoda , genetic divergence , zoology , genus , centipede , evolutionary biology , taxonomy (biology) , genetic diversity , gene , genetics , population , demography , sociology
The 680 bp fragment of the COI gene was employed for DNA barcodes of the millipede genus Tylopus in Vietnam. A total of 22 samples representing for 14 morphological Tylopus species were analyzed. The K2P genetic divergence between Tylopus species ranges from 12.2% to 18.9% with a mean of 15 ± 1%. The intraspecific divergences are slightly different between species, from 3% to 5%. The AGBD analysis and phylogenetic trees also support 14 morphological species. It is also suggested to have more COI sequences of more species for better barcode reference library and better molecular species identification.