Open AccessHIGH PERFORMANCE LIQUID CHROMATOGRAPHY ASSAY FOR DETERMINATION OF MOXIFLOXACIN IN HUMAN PLASMA
Author(s) -
Luong Thi My Hanh,
Nguyen Thi Minh Diep,
Thi Ngoc Mai Pham,
Nguyễn Xuân Trường
Publication year - 2019
Publication title -
vietnam journal of science and technology/science and technology
Language(s) - English
Resource type - Journals
eISSN - 2815-5874
pISSN - 2525-2518
DOI - 10.15625/2525-2518/57/3/13362
Subject(s) - moxifloxacin , chromatography , calibration curve , protein precipitation , high performance liquid chromatography , chemistry , acetonitrile , correlation coefficient , human plasma , analytical chemistry (journal) , detection limit , mathematics , antibiotics , biochemistry , statistics
A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.