Open AccessA Comparison of the Cytotoxic Activity of Extracts from Artificial Fruiting Bodies and Mycelial Biomass of Cordyceps neovolkiana (DL004) Fungus
Author(s) -
Dũng Chí Nguyễn
Publication year - 2018
Publication title -
vietnam journal of science and technology/science and technology
Language(s) - English
Resource type - Journals
eISSN - 2815-5874
pISSN - 2525-2518
DOI - 10.15625/2525-2518/56/4a/12946
Subject(s) - jurkat cells , cytotoxic t cell , sulforhodamine b , mycelium , ic50 , petroleum ether , fungus , chemistry , cytotoxicity , ethanol , traditional medicine , cell culture , biology , botany , in vitro , chromatography , biochemistry , extraction (chemistry) , t cell , immunology , medicine , immune system , genetics
C-HCTN-69Cordyceps neovolkiana is an insect-parasitic fungus, naturally distributed in the Langbiang mountain, Tam Dao and Ba Vi national parks of Vietnam. This study assessed and compared the in vitro cytotoxic activity of crude extracts from fruit bodies and biomass of artifical C. neovolkiana fungus against human Jurkat (acute T cell leukemia) and breast carcinoma (MCF-7) cells using the sulforhodamine B (SRB) assay. We obtained the extracts via the ethanol extraction and liquid-liquid extraction with four different solvents; ethanol (EtOH) petroleum ether (60 – 80oC) (PE), etyl acetate (ET), butanol (BU) and water (W), successively. The result shows both the cytotoxic potential of biomass and fruit-body extracts were determined the IC50 value at 0 – 100 µg/mL concentration. The PE-fruit-body extract displayed the highest cytotoxic activity on MCF-7 and Jurkat cell lines with IC50 values of 76,30 ± 1,20 µg/mL and 37,18 ± 1,39 µg/mL, respectively. Beside that, the PE- biomass extracts respectively showed the highest cytotoxic activity on MCF-7 and Jurkat cell lines with IC50 values of 26,94 ± 1,62 µg/mL and 15,50 ± 0,19. Obviously, we need to study further about the cytotoxic mechanism PE-fruit-body and biomass extracts on MCF-7 and Jurkat cells and chemical composition analysis of extracts to possibly use as a promising anticancer agent.