Open AccessSTUDY ON MIXING ANTI-A MONOCLONAL ANTIBODY AND ANTI-B MONOCLONAL ANTIBODY TO MAKE ANTI-AB SAVE AS ABO BLOOD GROUPING REAGENT
Author(s) -
Nguyễn Thị Trung,
Trương Nam Hải
Publication year - 2018
Publication title -
vietnam journal of science and technology/science and technology
Language(s) - English
Resource type - Journals
eISSN - 2815-5874
pISSN - 2525-2518
DOI - 10.15625/2525-2518/56/1/9372
Subject(s) - monoclonal antibody , abo blood group system , titer , agglutination (biology) , antibody , monoclonal , chemistry , microbiology and biotechnology , medicine , immunology , biology
So, it needs to balance the ratio of anti-A monoclonal antibody and anti-B monoclonal antibody in the mixing so that the possibility of agglutination is the best. In this paper, anti-A monoclonal antibody (titer is 1/256) and anti-B monoclonal antibody (titer is 1/256) was used. The best results were obtained at one volume anti A monoclonal antibody is mixed one volume anti-B monoclonal antibody. The anti-A,B antibody titer was 1/128 for red blood group A and it was 1/128 for red blood group B. The intensity of agglutination reached 3+ for both red blood group A and B.