Open AccessIsolation and characterization of the cellulose-degrading bacteria from Ngoc Linh ginseng soil in Quang Nam province
Author(s) -
Trần Bảo Trâm,
Phạm Hương Sơn,
Ngô Thị Hiền,
Ngô Thị Hoa,
Nguyễn Thu Hiền,
Nguyễn Huy Hoàng
Publication year - 2016
Publication title -
tạp chí công nghê sinh học
Language(s) - English
Resource type - Journals
ISSN - 1811-4989
DOI - 10.15625/1811-4989/14/1/9292
Subject(s) - bacteria , 16s ribosomal rna , ginseng , pseudomonas , biology , cellulose , botany , chemistry , biochemistry , medicine , genetics , alternative medicine , pathology
Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) is an endemic species in Vietnam and was discovered at the Ngoc Linh mountain (Kon Tum/Quảng Nam). Investigations showed that the soil with a thick layer of humus was the ideal condition for growth and development of Ngoc Linh ginseng. Therefore research on microbial flora as well as cellulose-degrading bacteria in ginseng soil may elucidate factors contributing to acclimatized cultivation of this ginseng in Vietnam. From the soil sample with cultivated Ngoc Linh ginseng in Quang Nam, five bacteria strains with cellulose-degrading activities were isolated (QN1, QN2, QN3, QN4, QN5 with respectively hydrolyzed CMC halos diameters of 10, 11, 22, 7, 22 mm) with cellulase activities of 1,31; 1,23; 2,99; 0,99; 2,51 U/ml. The combination of 16S rRNA gene sequences and cultured/biochemical characteristics of the bacteria showed that the five bacteria strains was classified to be Pseudomonas sp. QN1; Pseudomonas sp. QN4; Bacillus sp. QN2; Bacillus sp. QN3; Roseomonas sp. QN5.