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Ultrafast Dynamics of Photoactive Yellow Protein via the Photoexcitation and Emission Processes †
Author(s) -
Nakamura Ryosuke,
Hamada Norio,
Ichida Hideki,
Tokunaga Fumio,
Kanematsu Yasuo
Publication year - 2007
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/2006-06-23-ra-946
Subject(s) - photoexcitation , femtosecond , excited state , ultrashort pulse , spectroscopy , fluorescence , population , fluorescence spectroscopy , wavelength , ultrafast laser spectroscopy , photochemistry , femtochemistry , time resolved spectroscopy , pulse (music) , materials science , chemistry , atomic physics , optoelectronics , optics , physics , laser , demography , quantum mechanics , sociology , detector
Pump‐dump fluorescence spectroscopy was performed for photoactive yellow protein (PYP) at room temperature. The effect of the dump pulse on the population of the potential energy surface of the electronic excited state was examined as depletion in the stationary fluorescence intensity. The dynamic behavior of the population in the electronic excited state was successfully probed in the various combinations of the pump‐dump delay, the dump‐pulse wavelength, the dump‐pulse energy and the observation wavelength. The experimental results were compared with the results obtained by the femtosecond time‐resolved fluorescence spectroscopy.