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Cellular and Antitumor Activity of a New Diethylene Glycol Benzoporphyrin Derivative (Lemuteporfin) †
Author(s) -
Boch Ron,
Canaan Alice J.,
Cho Angela,
Dolphin David D.,
Hong Lina,
Jain Ashok K.,
North John R.,
Richter Anna M.,
Smits Claire,
Sternberg Ethan D.
Publication year - 2006
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/2005-06-03-ra-564
Subject(s) - photosensitizer , photodynamic therapy , in vivo , photosensitivity , chemistry , in vitro , diethylene glycol , phototoxicity , biophysics , pharmacology , photochemistry , biochemistry , materials science , medicine , ethylene glycol , biology , organic chemistry , microbiology and biotechnology , optoelectronics
A newly synthesized diethylene glycol functionalized chlorin‐type photosensitizer, lemuteporfin, was characterized for use in photodynamic therapy (PDT) in a panel of in vitro and in vivo test systems. The photosensitizer was highly potent, killing cells at low nanomolar concentrations upon exposure to activating light. The cellular uptake of lemuteporfin was rapid, with maximum levels reached within 20 min. Mitogen‐activated lymphoid cells accumulated more of the lemuteporfin than their quiescent equivalents, supporting selectivity. Photosensitizer fluorescence in the skin increased rapidly within the first few minutes following intravenous administration to mice, then decreased over the next 24 h. Skin photosensitivity reactions indicated rapid clearance of the photosensitizer. Intravenous doses as low as 1.4 μmol/kg combined with exposure to 50 J/cm 2 red light suppressed tumor growth in a mouse model. In conclusion, this new benzoporphyrin was found to be an effective photosensitizer, showing rapid uptake and clearance both in vitro and in vivo. This rapid photosensitization of tumors could be useful in therapies requiring a potent, rapidly accumulating photosensitizer, while minimizing the potential for skin photosensitivity reactions to sunlight following treatment.