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Time‐Resolved Emission Spectra of Green Fluorescent Protein
Author(s) -
Jaye Andrew A.,
StonerMa Deborah,
Matousek Pavel,
Towrie Michael,
Tonge Peter J.,
Meech Stephen R.
Publication year - 2006
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/2005-05-07-ra-518
Subject(s) - chromophore , green fluorescent protein , fluorescence , picosecond , spectral line , chemistry , emission spectrum , fluorescent protein , resolution (logic) , photochemistry , optics , physics , biochemistry , laser , astronomy , gene , artificial intelligence , computer science
The time‐resolved emission spectra of wild‐type green fluorescent protein (wtGFP) and the T203V GFP mutant have been recorded with picosecond time resolution, allowing the separate characterization of the two spectral components associated with the neutral and anionic forms of the GFP chromophore. Significantly, neither component shifts as a function of time. It is suggested that the absence of spectral shift is a result of highly restricted movement of the protein residues in the vicinity of the chromophore. The shapes of the separated spectra are discussed and their relative ratio analyzed in a steady‐state analysis.