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Novel Porphyrin Conjugates with a Potent Photodynamic Antitumor Effect: Differential Efficacy of Mono‐ and Bis‐β‐cyclodextrin Derivatives In Vifro and In Vivo
Author(s) -
Kralova Jarmila,
Synytsya Alla,
Pouckova Pavla,
Koc Michal,
Dvorak Michal,
Kral Vladimir
Publication year - 2006
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/2005-05-06-ra-516
Subject(s) - porphyrin , in vivo , cyclodextrin , conjugate , chemistry , photodynamic therapy , combinatorial chemistry , stereochemistry , pharmacology , biochemistry , organic chemistry , medicine , biology , mathematics , mathematical analysis , microbiology and biotechnology
In the present study we investigated the photosensitizing properties of two novel mono‐ and bis‐cyclodextrin tetrakis (pentafluorophenyl) porphyrin derivatives in several tumor cell lines and in BALB/c mice bearing subcutaneously transplanted syngeneic mouse mammary carcinoma 4T1. Both studied sensitizers were localized mainly in lysosomes and were found to induce cell death by triggering apoptosis in human leukemic cells HL‐60. In 4T1 and other cell lines both apoptotic and necrotic modes of cell death occurred depending on drug and light doses. Mono‐cyclodextrin porphyrin derivative P(β‐CD)l exhibited stronger in vitro phototoxic effect than bis‐cyclodextrin derivative P(β‐CD)2. However, in vivo P(β‐CD)2 displayed faster tumor uptake with maximal accumulation 6 h after application, leading to complete and prolonged elimination of subcutaneous tumors within 3 days after irradiation (100 J cm ‐2 ). In contrast, P(β‐CD)1 uptake was slower (48 h) and the reduction of tumor mass was only transient, reaching the maximum at the 12 h interval when a favorable tumor‐to‐skin ratio appeared. Thus, P(β‐CD)2 represents a new photosensitizing drug displaying fast and selective tumor uptake, strong antitumor activity and fast elimination from the body.