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Increased Intensities of YOYO‐1–labeled DNA Oligomers Near Silver Particles ¶
Author(s) -
Lakowicz Joseph R.,
Malicka Joanna,
Gryczynski Ignacy
Publication year - 2003
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2003)0770604iioydo2.0.co2
Subject(s) - oligomer , fluorescence , dna , substrate (aquarium) , chemistry , photochemistry , analytical chemistry (journal) , chromatography , polymer chemistry , biochemistry , biology , optics , ecology , physics
DNA detection is usually performed using fluorescence probes. Using a DNA oligomer stained with the widely used dye 1,1′‐[1,3‐propanediylbis[(dimethylimino)‐3,1‐propanediyl]]bis[4‐[(3‐methyl‐2(3H)‐benzoxazolylidene)methyl]]‐quinolinum tetraiodide (YOYO‐1), we show that a substrate containing silver particles can lead to a greater than 10‐fold increase in the fluorescence intensity. Proximity to silver particles also increases the photostability of YOYO‐1–DNA. These results suggest that substrates or gels containing silver particles may be used for increased sensitivity in DNA detection.