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Fluorescence Spectral Imaging of Dihydroxyacetone on Skin In Vivo ¶
Author(s) -
Forest Susan E.,
Grothaus Jeff T.,
Ertel Keith D.,
Rader Charlie,
Plante Janyl
Publication year - 2003
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2003)0770524fsiodo2.0.co2
Subject(s) - dihydroxyacetone , fluorescence , in vivo , chemistry , preclinical imaging , biophysics , photochemistry , biochemistry , biology , optics , physics , glycerol , microbiology and biotechnology
Dihydroxyacetone (DHA) has been proposed as a potential alternative to dansyl chloride for use as a fluorescence marker on skin to assess stratum corneum turnover time in vivo. However, the fluorescence from DHA on skin has not been adequately studied. To address this void, a noninvasive, noncontact spectral imaging system is used to characterize the fluorescence spectrum of DHA on skin in vivo and to determine the optimal wavelengths over which to collect the DHA signal that minimizes the contributions from skin autofluorescence. The DHA‐skin fluorescence signal dominates the 580–680 nm region of the visible spectrum when excited with ultraviolet radiation in the 320–400 nm wavelength region (UVA). An explanation of the time‐dependent spectral features is proposed in terms of DHA polymerization and binding to skin.