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Impairment of Eye Lens Cell Physiology and Optics by Broadband Ultraviolet A–Ultraviolet B Radiation ¶
Author(s) -
Oriowo O. M.,
Cullen A. P.,
Sivak J. G.
Publication year - 2002
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2002)0760361ioelcp2.0.co2
Subject(s) - ultraviolet , ultraviolet radiation , broadband , lens (geology) , optics , ultraviolet b radiation , eye lens , radiation , optoelectronics , physics , chemistry , radiochemistry
The phototoxicity of ultraviolet A (UVA) alone and UVA plus ultraviolet B (UVB) combined on cultured porcine lenses was investigated by analyzing cellular function as measured with a fluorescence bioassay approach and optical integrity, in terms of sharpness of the lens focus as measured with a scanning laser system. The bioassay consisted of carboxyfluorescein diacetate‐acetoxymethyl ester and alamarBlue fluorescent dyes. Aseptically dissected porcine lenses were maintained in modified medium 199 without phenol red supplemented with 1% penicillin–streptomycin and 4% porcine serum. At 1 week of preincubation, baseline measurements were obtained. Then the lenses were treated with single exposures of different UVA and UVB energy levels. The lenses treated with 86 J/cm 2 UVA alone showed a significant ( P < 0.05 ) decrease in cellular and optical integrity at 48 h after exposure, whereas those treated with 43 J/cm 2 UVA alone did not show significant phototoxic effect. Lenses treated with 15.63 J/cm 2 UVA plus 0.019 J/cm 2 UVB combined showed significant adverse effects beginning from 48 h after exposure. Also, there was no recovery. These findings show that a high UVA dose alone and relatively low UVA in combination with low UVB radiant exposure can impair lens cellular and optical functions, respectively.