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Assignment of UVB‐responsive cis ‐element and Protoplastization‐ (Dilution‐) and Elicitor‐responsive Ones in the Promoter Region of a Carrot Phenylalanine Ammonia‐lyase Gene ( gDcPAL1 ) ¶
Author(s) -
Takeda Junko,
Ito Yoshio,
Maeda Kazuhiro,
Ozeki Yoshihiro
Publication year - 2002
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2002)0760232aource2.0.co2
Subject(s) - elicitor , phenylalanine ammonia lyase , phenylalanine , dilution , chemistry , gene , biochemistry , ultraviolet light , peroxidase , microbiology and biotechnology , enzyme , biology , photochemistry , physics , amino acid , thermodynamics
Expression of a carrot phenylalanine ammonia‐lyase (PAL) gene ( gDcPAL1 ) in suspension‐cultured carrot cells is induced by dilution of the culture or by application of a fungal elicitor, as well as by ultraviolet B (UVB) irradiation. We demonstrated that among its upstream cis ‐elements (Takeda et al. [1997] Photochem. Photobiol. 66 , 464–470), L 4 is UVB responsive, and L 1 is protoplastization‐ (dilution‐) and elicitor responsive, from studies with transiently transformed mutated or truncated gDcPAL1 promoter– luc constructs. This conclusion is consistent with our observation that PAL activities induced by UVB and by protoplastization (dilution) or elicitor are additive.