Premium
Tuning Up or Down the UV‐induced Apoptosis in Chinese Hamster Ovary Cells with Cell Cycle Inhibitors ¶
Author(s) -
Tzang BorShow,
Chiang YunJung,
Lan HsinChieh,
Liao ChuBin,
Liu YinChang
Publication year - 2002
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2002)0750662tuodtu2.0.co2
Subject(s) - chinese hamster ovary cell , colcemid , cyclin dependent kinase , apoptosis , cell cycle , biology , microbiology and biotechnology , cyclin b1 , cdk inhibitor , cyclin dependent kinase 1 , cell culture , biochemistry , mitosis , genetics
Exposure to UVC induces apoptosis in Chinese hamster ovary (CHO.K1) cells. While studying the underlying mechanism, we found that a variety of cell cycle inhibitors, including colcemid, hydroxyurea and mimosine, enhance the UV‐induced apoptosis in these cells. Such enhancement was not dependent on the cell cycle progression nor was it related to the difference in UV sensitivity at different phases of the cell cycle. The expression of p21(waf1/cip1), a general cyclin‐dependent kinase (CDK) inhibitor, was deficient in CHO.K1 cells. Ectopic overexpression of the human p21 markedly increased the survival rates of the UV‐irradiated cells in the presence of colcemid. In addition, roscovitine, a small‐molecule inhibitor of CDK, also inhibited the UV‐induced apoptosis. These observations suggest that deregulation of CDK activity may be critical in the UV‐induced apoptosis in CHO.K1 cells.