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Modifications of In Vitro Skin Penetration Under Solar Irradiation: Evaluation on Flow‐through Diffusion Cells ¶
Author(s) -
Gélis Christelle,
Mavon Alain,
Delverdier Maxence,
Paillous Nicole,
Vicendo Patricia
Publication year - 2002
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2002)0750598moivsp2.0.co2
Subject(s) - penetration (warfare) , irradiation , in vitro , biophysics , diffusion , chemistry , materials science , biology , physics , thermodynamics , biochemistry , mathematics , operations research , nuclear physics
The effect of solar irradiation on ex vivo dermatomed hairless rat skin samples maintained in culture on flow‐through diffusion cells for at least 24 h was evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay and by histological observations. Transepidermal water loss (TEWL) measurements and kinetic analysis of the permeation of both tritiated water and 14 C caffeine through the skin were performed after full‐spectrum solar exposure involving the use of a xenon arc solar simulator. After a UV exposure of less than 420 mJ/cm 2 , skin integrity and permeation of both water and caffeine did not change significantly. In contrast, after a 420 mJ/cm 2 UV exposure, the epidermis appeared more contracted, associated with an increase of 55% of TEWL and 220% of the skin permeation of tritiated water after 6 h. The data suggested a dramatic alteration of the skin barrier integrity. Moreover, the flux of 14 C caffeine increased rapidly by 338% of the absorption of water 12 h after irradiation. These results reveal the presence of a threshold UV exposure that would not modify skin penetration.