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UVB‐activated Psoralen Reduces Luminal Narrowing After Balloon Dilation Because of Inhibition of Constrictive Remodeling ¶
Author(s) -
Perrée Jop,
Leeuwen Ton G.,
Velema Evelyn,
Smeets Mirjam,
Kleijn Dominique,
Borst Cornelius
Publication year - 2002
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2002)0750068uaprln2.0.co2
Subject(s) - dilation (metric space) , psoralen , balloon dilation , balloon , chemistry , cardiology , medicine , biochemistry , dna , mathematics , combinatorics
In this study we have explored the potential of PUVB (8‐MOP + UVB) therapy for the reduction of luminal narrowing after arterial injury. In 15 rabbits, balloon dilation of iliac arteries was performed. In 20 arteries, dilation was combined with the delivery of pulsed ultraviolet light B (UVB) irradiation with 10 arteries being previously subjected to sensitizer infusion. Changes in vessel diameter, proliferation and extracellular matrix protein content at 6 weeks were evaluated by means of angiography and histomorphometry–immunohistochemistry. We found that PUVB, applied at the time of dilation, induced reduction in late loss (LL) at 6 weeks (percutaneous transluminal angioplasty vs UVB vs PUVB: 0.64 ± 0.15 mm vs 0.61 ± 0.05 mm vs 0.29 ± 0.05 mm; p = 0.018). The same holds true for constrictive remodeling (0.53 ± 0.15 mm vs 0.45 ± 0.06 mm vs 0.15 ± 0.05 mm; p = 0.016). In the irradiation groups, LL was independent of acute gain (AG), as opposed to the control. Collagen content increased significantly after PUVB in media and adventitia, without increased cellular proliferation in all vessel layers. Thus, PUVB at the time of dilation reduced luminal narrowing at follow‐up without effecting proliferation. This effect was independent of AG and was associated with increased collagen content in media and adventitia.