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Photodynamic Effects Induced by Aminolevulinic Acid Esters on Human Cervical Carcinoma Cells in Culture ¶
Author(s) -
Xiang Wei,
Weingandt Helmut,
Ließmann Frank,
Klein Susanne,
Stepp Herbert,
Baumgartner Reinhold,
Hillemanns Peter
Publication year - 2001
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2001)0740617peibaa2.0.co2
Subject(s) - protoporphyrin ix , photosensitizer , photodynamic therapy , chemistry , kinetics , protoporphyrin , annexin , endogeny , apoptosis , fluorescence , in vitro , biochemistry , cervical carcinoma , microbiology and biotechnology , photochemistry , cancer , cervical cancer , porphyrin , biology , organic chemistry , physics , quantum mechanics , genetics
ABSTRACT Fluorescence diagnosis and photodynamic therapy using 5‐aminolevulinic acid (ALA) provide new methods for the detection and treatment of cervical cancer and especially its precursors. However, these techniques are restricted by the rate of uptake of the hydrophilic ALA, its poor diffusion through the bilayer of biological membranes or both. In this study we evaluated the effect of some esterified ALA derivatives on the induction of the endogenous photosensitizer, protoporphyrin IX (PpIX), and the photodamage in cultured human cervical cells (C33‐A and CaSki). The kinetics of PpIX accumulation showed that ALA esters, especially the ALA‐hexylester (h‐ALA), induced significantly faster PpIX formation than ALA at the same concentration (0.5 m M ). The PpIX induction showed a dose‐dependent characteristic. The highest PpIX values could be achieved by an up to 1.3–13‐fold lower concentration of ALA esters than with ALA. Using the Annexin V assay, apoptosis was found to be induced rapidly after irradiation in both ALA‐ and ALA esters–treated cells. On measuring mitochondrial activity, the incubation with h‐ALA induced a more pronounced photodamage. The results indicate that improved or at least comparable photodynamic effects can be achieved by using remarkably lower doses of ALA esters.