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Oligonucleotide‐conjugated Thiazole Orange Probes as “Light‐up” Probes for Messenger Ribonucleic Acid Molecules in Living Cells ¶
Author(s) -
Privat Eric,
Melvin Tracy,
Asseline Ulysse,
Vigny Paul
Publication year - 2001
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2001)0740532octopa2.0.co2
Subject(s) - chemistry , fluorescence , messenger rna , oligonucleotide , rna , internalization , biochemistry , microbiology and biotechnology , orange (colour) , dna , biology , gene , physics , quantum mechanics , cell , food science
“Light‐up” probes, icosa‐α‐thymidylate‐thiazole orange conjugates, for the in situ time‐resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa‐α‐thymidylate‐thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.