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The Application of a Compact Multispectral Imaging System with Integrated Excitation Source to In vivo Monitoring of Fluorescence During Topical Photodynamic Therapy of Superficial Skin Cancers ¶
Author(s) -
Hewett Jacqueline,
Nadeau Valerie,
Ferguson James,
Moseley Harry,
Ibbotson Sally,
Allen John W.,
Sibbett Wilson,
Padgett Miles
Publication year - 2001
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2001)0730278taoacm2.0.co2
Subject(s) - protoporphyrin ix , multispectral image , photodynamic therapy , autofluorescence , fluorescence , photosensitizer , in vivo , fluorescence lifetime imaging microscopy , human skin , optics , materials science , biomedical engineering , chemistry , medicine , computer science , photochemistry , physics , genetics , microbiology and biotechnology , organic chemistry , computer vision , biology
A novel, compact and low‐cost multispectral fluorescence imaging system with an integrated excitation light source is described. Data are presented demonstrating the application of this method to in vivo monitoring of fluorescence before, during and after topical 5‐aminolevulinic acid photodynamic therapy of superficial skin cancers. The excitation source comprised a fluorescent tube with the phosphor selected to emit broadband violet light centered at 394 nm. The camera system simultaneously captured spectrally specific images of the fluorescence of the photosensitizer, protoporphyrin IX, the illumination profile and the skin autofluorescence. Real‐time processing enabled images to be manipulated to create a composite image of high contrast. The application and validation of this method will allow further detailed studies of the characteristics and time‐course of protoporphyrin IX fluorescence, during topical photodynamic therapy in human skin in vivo.