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In vivo Near‐infrared Raman Spectroscopy: Demonstration of Feasibility During Clinical Gastrointestinal Endoscopy ¶
Author(s) -
Shim Martin G.,
Wong Kee Song LouisMichel,
Marcon Norman E.,
Wilson Brian C.
Publication year - 2000
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2000)0720146ivnirs2.0.co2
Subject(s) - raman spectroscopy , endoscope , in vivo , ex vivo , near infrared spectroscopy , gastrointestinal tract , endoscopy , optical fiber , spectroscopy , materials science , optics , biomedical engineering , pathology , chemistry , medicine , biology , radiology , physics , biochemistry , microbiology and biotechnology , quantum mechanics
Raman spectroscopy (RS) has potential for disease classification within the gastrointestinal tract (GI). A near‐infrared (NIR) fiber‐optic RS system has been developed previously. This study reports the first in vivo Raman spectra of human gastrointestinal tissues measured during routine clinical endoscopy. This was achieved by using this system with a fiber‐optic probe that was passed through the endoscope instrument channel and placed in contact with the tissue surface. Spectra could be obtained with good signal‐to‐noise ratio in 5 s. The effects on the spectra of varying the pressure of the probe tip on the tissue and of the probe‐tissue angle were determined and shown to be insignificant. The limited set of spectra from normal and diseased tissues revealed only subtle differences. Therefore, powerful spectral‐sorting algorithms, successfully implemented in prior ex vivo studies, are required to realize the full diagnostic potential of RS for tissue classification in the GI.