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Evaluation of the Protective Effect of Sunscreens on In Vitro Reconstructed Human Skin Exposed to UVB or UVA Irradiation
Author(s) -
Bernerd Françoise,
Vioux Corinne,
Asselineau Daniel
Publication year - 2000
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2000)0710314eotpeo2.0.co2
Subject(s) - photoprotection , pyrimidine dimer , sunburn , human skin , in vitro , chemistry , photoaging , irradiation , absorption (acoustics) , dermal fibroblast , biophysics , dermatology , fibroblast , materials science , biochemistry , dna damage , biology , medicine , dna , photosynthesis , genetics , physics , nuclear physics , composite material
We have previously shown that skin reconstructed in vitro is a useful model to study the effects of UVB and UVA exposure. Wavelength‐specific biological damage has been identified such as the formation of sunburn cells (SBC) and pyrimidine dimers after UVB irradiation and alterations of dermal fibroblasts after UVA exposure. These specific effects were selected to evaluate the protection afforded by two sunscreens after topical application on the skin surface. Simplified formulations having different absorption spectra but similar sun protection factors were used. One contained a classical UVB absorber, 2‐ethylhexyl‐ p ‐methoxycinnamate. The other contained a broad‐spectrum absorber called Mexoryl® SX, characterized by its strong absorbing potency in the UVA range. Both filters were used at 5% in a simple water/oil vehicle. The evaluation of photoprotection on in vitro reconstructed skin revealed good efficiency for both preparations in preventing UVB‐induced damage, as shown by SBC counting and pyrimidine dimer immunostaining. By contrast, only the Mexoryl® SX‐containing preparation was able to efficiently prevent UVA‐specific damage such as dermal fibroblast disappearance. Our data further support the fact that skin reconstructed in vitro is a reliable system to evaluate the photoprotection provided by different sunscreens against specific UVB and UVA biological damage.