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Effects of Cytosine Methylation on Pyrimidine Dimer Formation in DNA
Author(s) -
Mitchell David L.
Publication year - 2000
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2000)0710162eocmop2.0.co2
Subject(s) - pyrimidine dimer , cytosine , pyrimidine , dna , chemistry , dna methylation , photochemistry , cyclobutane , dimer , irradiation , duplex (building) , methylation , dna damage , microbiology and biotechnology , biochemistry , biology , ring (chemistry) , gene , gene expression , organic chemistry , physics , nuclear physics
The relative induction of cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4)pyrimidone photoproducts ([6–4]PD) was quantified in the duplex homopolymers polydeoxyadenosine : polydeoxythymidine, polydeoxyguanosine : polydeoxycytidine and polydeoxyguanosine :polydeoxy‐5‐methylcytidine irradiated with UVC or UVB radiation. Cytosine methylation significantly increased the yield of cytosine (6–4)PD after irradiation with UVC light and of cytosine CPD and (6–4)PD after irradiation with UVB light. The data suggest that CPD and (6–4)PD are preferentially induced at 5‐methylcytosine bases in DNA of cells exposed to sunlight and comprise a major component of the mutation spectrum leading to the initiation of sunlight‐induced skin cancer.