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Suppression of sialylated by sulfated oligosaccharides in negative MALDI‐FTMS
Author(s) -
An Hyun Joo,
Lebrilla Carlito B.
Publication year - 2001
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1560/n390-tjrb-u607-fqpc
Subject(s) - chemistry , oligosaccharide , sulfation , mass spectrometry , matrix assisted laser desorption/ionization , chromatography , biochemistry , desorption , organic chemistry , adsorption
Sialylated oligosaccharides are suppressed by sulfated oligosaccharides during matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry. The sialylated oligosaccharide may be suppressed by as much as a factor of ten to twenty. This suppression factor is constant over a large concentration range. As both sialylated and sulfated (or anionic) oligosaccharides are found together in many biological systems, the suppression has important consequences in the analysis of these compounds. Methods for overcoming the effect of suppression without laborious separation are provided.