Open AccessProductivity of Marine Archaea Haloferax mediterranei Culture in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production with Yeast Extract as Nutrient Source
Author(s) -
Susiana Melanie,
James Winterburn
Publication year - 2017
Publication title -
squalen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 1
eISSN - 2406-9272
pISSN - 2089-5690
DOI - 10.15578/squalen.v12i3.318
Subject(s) - polyhydroxyalkanoates , biodegradable plastic , raw material , biomass (ecology) , food science , pulp and paper industry , yeast extract , chemistry , fermentation , nutrient , archaea , biodegradation , biology , bacteria , organic chemistry , biochemistry , ecology , gene , genetics , engineering
Currently, most of household goods are made from polymer or plastic. The polymer manufacturing industry has been relying on fossil fuel-based raw materials which is non-biodegradable. Plastics derived from these non-biodegradable sources will be difficult to degrade in nature, which in turn will pollute the environment and harm the ecosystem. One way to reduce the ecosystem damages caused by plastic made from petroleum-based plastic is to use biodegradable materials as plastic industry raw materials, such as Polyhydroxyalkanoates (PHA). Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV is an example of PHA that can be produced by several types of microorganisms, such as marine archaea Haloferax mediterranei. This objective of this study is to determine the productivity of marine archaea cultures H. mediterranei to produce PHBV with yeast extract as main nutrient sources. H. mediterranei will produce PHBV at extreme conditions with excess carbon and limited nutrients. Experiments and analysis were conducted in triplicate and batch operating systems. During the fermentation process, Optical Density, glucose levels, phosphorus levels, pH, and Dry Cell Weight (DCW) were observed. The final product then analysed using GC-MS to determine the PHBV content and 3HB (3-hydroxybutyrate) and 3HV (3-hydroxyvalerate) fraction composition in the PHBV. The results show that the maximum specific growth rate and doubling time (Td) of the H. mediterranei were 0.1258 hours-1 and 5.51 hours. H. mediterranei biomass were increasing until it reached stationary phase at 95 hours. The results show PHBV content, dry biomass and yield PHA to substrate were 2.62 g/L, 29.1% and 0.27 g/g.