Open AccessMETHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE
Author(s) -
N. Pirovski,
Angel Yordanov,
Dimitrinka Atanasova
Publication year - 2019
Publication title -
trakia journal of sciences
Language(s) - English
Resource type - Journals
eISSN - 1313-3551
pISSN - 1312-1723
DOI - 10.15547/tjs.2019.s.02.016
Subject(s) - tongue , acupuncture , fixation (population genetics) , acupuncture needle , consistency (knowledge bases) , sagittal plane , protocol (science) , medicine , biomedical engineering , computer science , orthodontics , anatomy , pathology , artificial intelligence , population , alternative medicine , environmental health
Changes occurring in acupuncture and sublingually administration of medicines also have a morphological basis, which can be objectively assessed. Objective: Refine of the methodology for evaluating morphological changes associated with tongue acupuncture. Material and methods: Selection of experimental animals and appropriate protocol for material collection, fixation, staining and counting of selected morphological structures and elements. Results: The samples fixed with formalin 10% were fixed well, the consistency of the tissues remained hard and the cuts were obtained well in all thicknesses (from 5 to 15 μm). Cuts from 5 to 7 μm are suitable for visualizing the structure of the tongue. The sagittal cuts provide the largest area for single-cut exploration, as well as are more suitable than others to track the acupuncture channel in the chosen for our study point. Conclusions: The study can be carried out in rats, but if possible, it is preferable to take the material from pigs. The use of formalin 10% gives a better texture to the material and facilitates the cutting with the microtome. It is necessary to use a variety of colorings due to the wide range of structures that are involved with the acupuncture needle tract.